![]() Neither the P 1 nor the F 1 mutant fish showed any noticeable changes in in body weight, survival rate, and hatching rate when the reproductive gene was knocked out. Polymerase chain reaction (PCR) identification showed no potential TALEN plasmid integration into the P 1 channel catfish genome. ![]() After confirmation of the low fertility of P 1 mutants in 2016, human chorionic gonadotropin (HCG) hormone therapy improved the spawning rates by 80% for female mutants and 88.9% for male mutants, and the mean hatch rate was 35.0% for F 1 embryos, similar to that of the controls ( p > 0.05). Without hormone therapy, the spawning rates of P 1 mutants ranged from 33.3 to 40.0%, with an average egg hatching rate of 0.75%. Ten of fifteen (66.7%) control pairs spawned, and their eggs had 32.3–74.3% average hatch rates in 20. Targeted fsh cleavage efficiency was 63.2% in P 1 fsh-knockout catfish. In this study, transcription activator-like effector nuclease (TALEN) plasmids targeting the fsh gene were electroporated into fertilized eggs with the standard double electroporation technique. ![]() Knocking out the fsh gene in the fish zygote should suppress the reproduction of channel catfish ( Ictalurus punctatus). Follicle-stimulating hormone ( fsh) plays an important role in sexual maturation in catfish. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |